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Scientific reference of in-vitro fermentation assay for human

Scientific reference of in-vitro fermentation assay for human

Article title: “Valorization of Brewer’s spent grain to prebiotic oligosaccharide: Production, xylanase catalyzed hydrolysis, in-vitro evaluation with probiotic strains and in a batch human fecal fermentation model”

Authors: Mursalin Sajib, Peter Falck, Roya R.R. Sardari, Sindhu Mathew, Carl Grey, Eva Nordberg Karlsson, Patrick Adlercreutz

Journal of Biotechnology (2018) 268, 61-70

Biotechnology, Department of Chemistry, Lund University (Sweden)

Gas Endeavour® was used as an automated in-vitro fermentation system in the establishment of a batch fermentation model using human fecal cultures. The duration of in-vitro test lasts over 48 hours and the volume of gas released from the fermentation can be monitored continuously for kinetic data analyses.