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Batch in-vitro digestibility assay for ruminants and monogastric animals

For the purpose of feed and food evaluation, in-vitro digestion and fermentation methods are ethically superior, faster and less expensive than in-vivo techniques. A good example of an in-vitro fermentation method is the in-vitro gas production technique, in which the gas evolved as a result of fermentation is used as the primary measurement. The method utilises the relationship between degradation and fermentative gas production to evaluate the nutritional parameters of the feed. Although the gas production technique is well-established in the area of ruminant feed evaluation, reports can also be found for nutritive evaluation of feed for monogastric animals and food evaluation for human. The benefits of in-vitro gas production techniques for digestibility evaluation include being able to run large batches simultaneously at a low cost, the ability to measure fermentation kinetics of soluble and insoluble fractions of feed or food, and the ability to easily make relative comparisons among different samples.

The Gas Endeavour® is our premier automatic instrument for continuous monitoring of fermentation gas (i.e., hydrogen, methane, carbon dioxide) released in in-vitro ruminant digestive models and in-vitro monogastric hindgut digestive model for monogastric animals and human. In comparison with the other in-vitro methods measuring the transformation of fermentable substrate, the Gas Endeavour® allows highly accurate analysis for a large number of samples in a short time. In addition, the automated in-vitro protocol based on the Gas Endeavour® significantly reduces the workload compared with manual analyses.

Simplify the analysis of feed and food quality

Areas of application

related to in-vitro gas production analysis

Gas Endeavour® is a powerful analytical tool to predict the rumen organic matter degradation through provision of kinetic information. Examples of application include estimating the energy content of feedstuffs for diet formulation, optimising efficiency of feed utilization, ruminant output, assessing bioactive components with antimethanogenic properties as well as antinutritive factors.

Like the rumen, the large intestine of simple-stomached animals is essentially a fermentation chamber where material is degraded by gut bacteria. The cumulative gas production technique can also be applied here and Gas Endeavour® can be an ideal batch test platform of in-vitro hindgut digestion to investigate differences among feedstuffs, unweaned and adult animals and also the effect of enzymatic pre-treatment on fermentation kinetics.

The fermentation of dietary fibers by gut microbiota results in the generation of gas and production of short-chain fatty acids (SCFA), and can also provide selective substrates for growth of specific groups of bacteria that may enhance the intestinal health of the host. Gas Endeavour® is an automated batch test platform of in-vitro lower gastrointestinal digestion and human fecal fermentation for studying microbiota population in lower gastrointestinal tract and fermentation properties of various dietary fibers.

benefits & advantages

Highly precise and accurate data

The Gas Endeavour® gives you a better understanding of the digestion kinetics of fermentable substrates. The precision of measurement and data calculations have been validated with the highest quality and standards by scientists in international inter-laboratory studies.

Significant reduction in time consumption and labour requirements

Fully automated analytical procedures, extendable testing capacity and full control of experiments with remote access. The Gas Endeavour® reduces the time consumption and labour requirement for in-vitro digestibility analysis and make the test being less skill dependent for precise and accurate data.

Standardised measurement procedures, data interpretations and reports

Real-time temperature and pressure compensations minimise the impact of possible variation in measurement conditions in analysis and standardise data presentation satisfying the highest demands for data accuracy and precision. An adaptive nonlinear mathematical model is also implemented in the latest released Gas Endeavour® to achieve an outstanding high linearity for in-vitro gas production analysis in all measurement ranges.

Compact and modular design

The modular approach enables flexible system set-up, easy upgrading options and simple maintenance. The Gas Endeavour® can easily be further expanded by connecting multiple instruments with a network switch in order to satisfy demands and shorten development times.

User-friendly operations with remote access

Simple to use and easy to learn. The web-based software application makes setting up and monitoring experiments very easy. The Gas Endeavour® allows easy access from a remote location using any computer, smartphone or tablet.

 – a novel tool for in-situ digestibility analysis

The Gas Endeavour® is a novel platform for analysing low gas volume and flow whenever there is a demand for high accuracy and precise measurements. The instrument can be used for research and industrial applications relating to: animal nutrition, wastewater, bioethanol fermentation, aerobic and anaerobic respiration, greenhouse gas emission, evaluation of microbial communities and more.

For more information, you are welcome to visit the Gas Endeavour page or download the Gas Endeavour brochure on in-vitro digestibility below.

Reference

Scientific reference on in-vitro digestibility for monogastrics

Article title: “Investigating the potential of different dietary fibers to simulate butyrate production in vitro

Author: Pierre Celestin Muragijeyezu
Thesis (2020), Swedish University University of Parma (Italy), Department of Medicine and Veterinary Sciences

Gas Endeavour® was used as an automated in-vitro system for evaluate the potential of dietary fibres to simulate butyrate production in the gut of pigs (hindgut fermentation in monogastric animals). The in-vitro test duration often lasts 24 hours.

Reference

Scientific reference on in-vitro digestibility assay for ruminants

Article title: “Evaluation of additives capable to improve ruminal fermentations through the use of an automated gas production system”

Athour: B. Matteo
Thesis (2020), University of Parma (Italy), Department of Medicine and Veterinary Sciences

Gas Endeavour® was used as an automated gas measuring system for conducting an in-vitro digestibility study for feed additives in ruminal fermentation. The instrument allows simultaneously simulating and continuously monitoring the physiological rumen movements in 15 testing flasks using a substrate quantity of 2-8 g dry sample or about 20 grams of fresh forage, depending on the intrinsic characteristics of each substrate.

Reference

Scientific reference of in-vitro fermentation assay for human

Article title: “Valorization of Brewer’s spent grain to prebiotic oligosaccharide: Production, xylanase catalyzed hydrolysis, in-vitro evaluation with probiotic strains and in a batch human fecal fermentation model”

Authors: Mursalin Sajib, Peter Falck, Roya R.R. Sardari, Sindhu Mathew, Carl Grey, Eva Nordberg Karlsson, Patrick Adlercreutz

Journal of Biotechnology (2018) 268, 61-70

Biotechnology, Department of Chemistry, Lund University (Sweden)

Gas Endeavour® was used as an automated in-vitro fermentation system in the establishment of a batch fermentation model using human fecal cultures. The duration of in-vitro test lasts over 48 hours and the volume of gas released from the fermentation can be monitored continuously for kinetic data analyses.

BPC Instruments AB staff

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